mouse anti pp1α  (Proteintech)

Journal: Cell Reports Methods

Article Title: Combination of in vivo and in vitro phosphoproteomics determines the PP2A target repertoire on proteome scale

doi: 10.1016/j.crmeth.2025.101084

Figure Lengend Snippet: Global phosphoproteome-wide in vitro phosphatase assays of PP1 and PP2A (A) Volcano plot highlighting identified phosphatase subunits in StrepHA-PPP1CA (right) and StrepHA-PPP2CA (left) purifications (marked in red) under native conditions. Dotted lines mark significance threshold; significantly enriched proteins marked in blue ( n = 3 biological replicates, FDR <0.05). Significantly enriched phosphatase subunits are annotated and highlighted as protein-interaction networks using information present in STRING DB. (B) Differentially regulated PPP1CA/PPP2CA sites, compared to either untreated control sample (left), or phosphatase- and okadaic acid-treated samples (OA, right). In vitro significantly regulated sites are highlighted in blue ( n = 3 biological replicates; FDR <0.05). (C and D) Motif and preference analyses of PPP1CA- and PPP2CA-sensitive sites. In motif analyses phosphatase-sensitive and OA-sensitive and insensitive sites are compared. Coloring corresponds to biochemical properties of residues. In preference analyses, the relative abundance of a given amino acid in a given position is indicated comparing phosphatase- and OA-sensitive (fold change of ≥3) to phosphatase-insensitive phosphosites. Colored lines next to amino acid names signify amino acid characteristics: green for hydrophobic, blue for basic, red for acidic, and gray for neutral amino acids. See also .

Article Snippet: Mouse monoclonal anti PPP1CA , Santa Cruz Biotech , Cat# sc-7482; RRID: AB_628177.

Techniques: In Vitro, Control

Journal: Cell Reports Methods

Article Title: Combination of in vivo and in vitro phosphoproteomics determines the PP2A target repertoire on proteome scale

doi: 10.1016/j.crmeth.2025.101084

Figure Lengend Snippet: Characterization of bona fide PP2A-PPP2R5E target sites (A) Complexome profiling of StrepHA-PPP2R5E purifications indicates enrichment of holocomplexes. (B) Volcano plot highlighting identified phosphatase subunits in StrepHA- PPP2R5E (left) and StrepHA-PPP1CA (right) purifications (marked in red) under native conditions. Dotted lines mark significance threshold, significantly enriched proteins marked in blue ( n = 3 biological replicates, FDR <0.05). Significantly enriched phosphatase subunits are annotated. (C) Significantly regulated phosphosites for PPP2R5E-PP2A complexes being OA sensitive ( n = 3 biological replicates). Significant sites are highlighted in blue (FDR <0.05). (D) Comparison of OBIPhA and in vivo analyses. Venn diagram highlights the overlap of identified phosphosites using the different experimental approaches. Bona fide PPP2R5E-PP2A target sites are defined as being regulated in vivo and in vitro (i.e., 194 sites on 168 proteins). (E) SLiMs in the bona fide PPP2R5E/B56ε-PP2A targets. (F) GO enrichment analysis using STRING DB highlighting biological processes of PPP2R5E-PP2A target proteins. (G) Targeted, phosphosite-specific MS analysis (PRM) for DDX3X Ser90 and Ser609. Shown is the quantification of three replicates (black dots). ∗ p < 0.05; ∗∗ p < 0.01; t test. (H) IF of CAPRIN1 and G3BP1. Shown are exemplary images of n = 3 biological replicates. Nuclei are stained in blue. Scale bar, 10 μm. Box plots show quantifications of 12 images of n = 3 biological replicates, boxes are drawn according to ggplot2 standard settings. White dots indicate average stress granule number/mean volume per cell and image. ∗ p < 0.05; ∗∗ p < 0.01; t test. See also .

Article Snippet: Mouse monoclonal anti PPP1CA , Santa Cruz Biotech , Cat# sc-7482; RRID: AB_628177.

Techniques: Comparison, In Vivo, In Vitro, Phospho-proteomics, Staining